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Institute Of Infectious Disease and Molecular Medicine

Institute Of Infectious Disease and Molecular Medicine

Core Facilities. Equipment. IDM Extension Project. Services. Resources for Member Groups. More. Calendar. News. For information on South Africa's response to COVID-19 please visit the COVID-19 Corona Virus South African Resource Portal.

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Principles and potential of the anaerobic ... - ScienceDirect

Principles and potential of the anaerobic ... - ScienceDirect

Dec 01, 2008 AD is a complex process which requires strict anaerobic conditions (oxidation reduction potential (ORP)−200 mV) to proceed, and depends on the coordinated activity of a complex microbial association to transform organic material into mostly CO 2 and methane (CH 4).Despite the successive steps, hydrolysis is generally considered as rate limiting , , , , , .

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Tuning PFKFB3 Bisphosphatase Activity Through Allosteric ...

Tuning PFKFB3 Bisphosphatase Activity Through Allosteric ...

Dec 30, 2019 Ammonium formate, fructose-6-phosphate (F6P), Tris HCl, glycerol, 2-mercaptoethanol, sodium phosphate and Amicon-ultra centrifugal filters (30 kDa) were purchased from Sigma Aldrich.

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Chemogenetic Control of Nanobodies Nature Methods

Chemogenetic Control of Nanobodies Nature Methods

Feb 17, 2020 GFP LAMAs can be used to control the localization of other family members of GFP ... 27 and contains a Klenow Polymerase fill in at the ... dye was removed by centrifugal filter units (Amicon).

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Identification functional characterization and crystal ...

Identification functional characterization and crystal ...

For crystallization, buffer of the protein solution was changed to 25 m m Tris-HCl (pH 7.5) using Amicon Ultra-4 centrifugal filter devices, 30,000 MWCO (Millipore, Billerica, MA). Crystals of PpLGDH were grown at 20 C using the hanging drop vapor diffusion method by mixing 0.5 μl of a 10 mg/ml protein solution with an equal volume of a ...

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Log In to Canvas

Log In to Canvas

Forgot Password? Enter your TMCC User Name and we'll send you a link to change your password.

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Enzyme leaps fuel antichemotaxis - PNAS

Enzyme leaps fuel antichemotaxis - PNAS

Jan 02, 2018 Challenging the traditional view that enzyme kinetics are only a matter of catalyzing chemical reactions, there is mounting evidence that the enzyme catalysis enhances enzyme mobility. This is significant to programming spatio-temporal patterns of molecular response to chemical stimulus, which is common to living matter as well as to significant …

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Simultaneous evaluation of multiple microarray surface ...

Simultaneous evaluation of multiple microarray surface ...

Jan 04, 2020 Surface chemistry is a crucial aspect for microarray modality biosensor development. The immobilization capability of the functionalized surface is indeed a limiting factor for the final yield of the binding reaction. In this work, we were able to simultaneously compare the functionality of protein ligands that were locally immobilized on different polymers, while on the …

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Butelase 1 is an Asx-specific ligase enabling peptide ...

Butelase 1 is an Asx-specific ligase enabling peptide ...

Jul 20, 2014 An example of this type of ring is the family of θ ... using 10-kDa cutoff centrifugal filter units (Amicon Ultra, Millipore). ... 100 μg/ml to …

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Coordinated DNA and histone dynamics drive accurate ...

Coordinated DNA and histone dynamics drive accurate ...

Mar 09, 2022 The purified DNA was concentrated to 10 μM in a 0.5-ml Amicon centrifugal filter unit with 10-kDa cutoff (Thermo Fisher Scientific) and stored at −20C. The 233-bp 80-N-6 and 6-N-80 DNA constructs labeled with Cy5 were generated by PCR using the appropriate Cy5-labeled primers as listed in tables S2 and S3.

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Welcome to Madison County OH

Welcome to Madison County OH

Mar 26, 2022 Welcome to Madison County, Ohio. Your source for Official Madison County Government Information. Named after James Madison, 4th President of the United States, Madison County is located in central Ohio between Columbus and Springfield. Primarily an agricultural area, 88% of the land consists of farms. Madison County ranks fourth in both …

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Legionella effector AnkX displaces the switch II region ...

Legionella effector AnkX displaces the switch II region ...

May 15, 2020 Excess of nucleotide was separated by exchanging the buffer three times with adduct buffer [20 mM tris, 300 mM NaCl, 5% (v/v) glycerol, and 1 mM β-ME (pH 8.0)] using Amicon Ultra 15-ml centrifugal filters (Merck Millipore). The purified binary conjugate was incubated with His 10-PreScission-Rab1b at a molar ratio of 1:1 overnight at 20C.

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Alginate/chitosan nanoparticles for ... - ScienceDirect

Alginate/chitosan nanoparticles for ... - ScienceDirect

Nov 01, 2014 The separation was performed using an Amicon Ultra-0.5 centrifugal filter device (Amicon Ultra – 0.5 mL 3 K device, Millipore Corp., Ireland). Briefly, 0.5 mL of sample was added to the Amicon and was centrifuged at 14,000 g during 10 min. After centrifugation a filtrate with free vitamin and a concentrate with nanoparticles with ...

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Amilorides inhibit SARS-CoV-2 replication in vitro by ...

Amilorides inhibit SARS-CoV-2 replication in vitro by ...

Nov 26, 2021 After desalting with a Millipore Amicon Ultra-4 centrifugal filter, the RNAs were annealed by heating for 2 min at 95C and flash cooled on ice. The samples were thoroughly washed and concentrated down with a 100% D 2 O buffer of …

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Cryo-EM structures reveal distinct mechanisms of ... - PNAS

Cryo-EM structures reveal distinct mechanisms of ... - PNAS

Oct 05, 2020 ABCB1, also known as P-glycoprotein or MDR1, is an ATP-binding cassette (ABC) transporter expressed at several blood–organ barriers, where it extrudes a wide spectrum of xenobiotic compounds outside the cell, thereby protecting tissues from toxic substances (1, 2).This is pharmacologically important because it reduces uptake of certain orally administered …

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Review Precision Hs Stock [T8ZA4N]

Review Precision Hs Stock [T8ZA4N]

Remington 700 PST007 Stock HS Precision. 99 at MidwayUSA, and are priced at $350. This rifle features a 20 stainless steel barrel and the addition of an HS Precision stock gives you a rock solid shooting platform. This is a review of the HS Precision Pro Series rifle, detail photos of the rifle and targets.

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Human cervicovaginal mucus contains an activity that ...

Human cervicovaginal mucus contains an activity that ...

Sep 19, 2012 The nanoparticles were purified by ultracentrifugation using 10 kDa cutoff centrifugal filters (Millipore Amicon, Billerica, MA) by washing 5 with deionized water until the filtrate was absent ...

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Copyright Notice

Copyright Notice

The following elements must be included in your copyright infringement complaint notice: 1. An electronic or physical signature of the copyright owner …

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xkcd: Graphic Designers

xkcd: Graphic Designers

This work is licensed under a Creative Commons Attribution-NonCommercial 2.5 License. This means you're free to copy and share these comics (but not to sell them). More details..

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