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after centrifugation the supernatant was discarded ...

after centrifugation the supernatant was discarded ...

after centrifugation the supernatant was neutralized. after centrifugation the supernatant was decanted. after preincubation the supernatant was discarded. after centrifugation the supernatant was loaded. after centrifugation the supernatant was filtrated. after centrifugation the supernatant was kept.

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after centrifuging the supernatant was discarded English ...

after centrifuging the supernatant was discarded English ...

After centrifugation, the supernatant was discarded and 1 ml of growth medium was added, triturated and centrifuged for 5 min at 400 rpm. After centrifugation, the supernatant was discarded and the pellet was immediately resuspended in fresh PBS, gently shaken, and centrifuged as before.

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Isolation of mitochondria from cells and tissues - PMC

Isolation of mitochondria from cells and tissues - PMC

After low speed centrifugation to remove nuclei and unbroken cells, the resulting supernatant is filtered with gauze and centrifuged twice at high speed. Finally, the …

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Plasma Supernatant - an overview ScienceDirect Topics

Plasma Supernatant - an overview ScienceDirect Topics

After red blood cell sedimentation, but before formation of a buffy coat, the supernatant plasma, containing white blood cells and platelets, is removed. If purified suspensions of lymphocytes are used, the cells are collected by centrifugation after the purification procedure and washed as described below.

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Sample Processing and Storage - Center for Research in ...

Sample Processing and Storage - Center for Research in ...

After using a mechanical homogenizer, sonicate the homogenate for 60 seconds, to ensure cell disruption. Centrifuge the tissue homogenate to remove cell debris. After centrifugation, remove the tissue homogenate supernatant and place it into a polypropylene microcentrifuge tube or a 12 X 75 polypropylene tube.

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What is cell culture supernatant? - FAQS.TIPS

What is cell culture supernatant? - FAQS.TIPS

Andrew J Spiers Popular answer. Dear Jonas, I think the meaning of 'cell culture supernatant' is fairly easily defined if you don't want to think too far - it is simply the fluid (and suspended solids including some cells) after centrifugation under particular …

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What does the supernatant contain?

What does the supernatant contain?

Apr 08, 2020 supernatant. (Science: chemistry) The soluble liquid fraction of a sample after centrifugation or precipitation of insoluble solids. Also know, what is a supernatant solution? The supernatant is the clear liquid that lies above the solid residue after centrifugation, precipitation, crystallization or settling. The liquid is normally free of precipitate and has a lower density.

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Centrifugation of bloody fluid - eClinpath

Centrifugation of bloody fluid - eClinpath

Centrifugation of bloody fluid. After centrifugation, an opaque light to medium red effusion has a clear yellow supernatant and a red pellet. This indicates the presence of red blood cells, which are causing the cloudiness. The fluid may or may not have an increased nucleated cell count. Previous Image Next Image.

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Centrifugation - an overview ScienceDirect Topics

Centrifugation - an overview ScienceDirect Topics

Centrifugation. After centrifugation, introduce the centrifuge tubes back into the anaerobic chamber and carefully decant the supernatant into a clean glass vessel. From: Methods in Enzymology, 2012. Related terms: Monospecific Antibody; pH; Bacterium; Filtration; Cell Lysate; Supernatant; Purification

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Differential Centrifugation Principle Protocol Uses

Differential Centrifugation Principle Protocol Uses

Dec 31, 2021 After centrifugation, the soluble cytosolic proteins are placed in the supernatant whereas membrane-associated proteins will be found in the more dense pellet. To further enhance proteins from an organelle in the cellular system …

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supernatant Example sentences

supernatant Example sentences

Examples of how to use “supernatant” in a sentence from the Cambridge Dictionary Labs

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Which Organelle Would Pellet First During Centrifugation

Which Organelle Would Pellet First During Centrifugation

Feb 08, 2022 When you use the centrifuge the first time where does the DNA go? After centrifugation, all the cell debris has been forced to the bottom of the PCR tube (1), leaving only the DNA in the liquid supernatant (2). The supernatant should look clear, like water. Finally, you will transfer the supernatant into a new PCR using the micropipette.

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Why is supernatant important? -

Why is supernatant important? -

Feb 26, 2020 The supernatant is the clear liquid that lies above the solid residue after centrifugation, precipitation, crystallization or settling. The liquid is normally free of precipitate and has a lower density.

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Fermented milk retains beneficial effects on skeletal ...

Fermented milk retains beneficial effects on skeletal ...

Fermented milk retains beneficial effects on skeletal muscle protein anabolism after processing by centrifugation and supernatant removal J Dairy Sci. 2021 Feb;104(2):1336-1350. doi: 10.3168/jds.2020-18780. Epub 2020 Nov 25. Authors K Sumi 1 ...

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Q: After centrifugation of milk the supernatant is ...

Q: After centrifugation of milk the supernatant is ...

Home; Dairy Engineering Advanced; Dairy Engineering Centrifugal Seperators; Question: After centrifugation of milk, the supernatant is? Options. A : Fat

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After centrifugation of milk the supernatant is ...

After centrifugation of milk the supernatant is ...

Multiple Choice Questions /. Agricultural Engineering MCQs /. Dairy Engineering Mcqs /. Question. Question Answer. Q: After centrifugation of milk, the …

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MCQ for Isolation of DNA - Chinese University of Hong Kong

MCQ for Isolation of DNA - Chinese University of Hong Kong

Question 3: After centrifugation, the supernatant being pipetted out contains ____ Water. INCORRECT. Please try again. DNA . CORRECT. The supernatant is DNA extracted from cells. Cellular debris. INCORRECT. Cellular debris is at the bottom of the tube. All of them. INCORRECT. Only one of them is the correct answer.

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Removing the Supernatant - San Diego Miramar College

Removing the Supernatant - San Diego Miramar College

Removing the supernatant by pouring off the supernatant. The supernatant is the solution above the solid that has been forced to the bottom of the centrifuge tube. To remove the supernatant, carefully pour or pipette the solution away from the solid. If the solid becomes re-suspended as the supernatant is removed, centrifuge the sample again.

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Preanalytics of urine sediment examination: effect of ...

Preanalytics of urine sediment examination: effect of ...

Results: The number of erythrocytes, leukocytes and squamous epithelial cells was significantly lower after centrifugation at 400xg (P = 0.001, 0.002 and 0.004, respectively). The number of leukocytes was significantly lower in conical tubes (P = 0.010), after the suction of supernatant (P = 0.045) and in 5 mL urine (P 0.001).

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(PDF) The effect of supernatant product of adipose tissue ...

(PDF) The effect of supernatant product of adipose tissue ...

The effect of supernatant product of adipose tissue derived mesenchymal stem cells and density gradient centrifugation preparation methods on pregnancy in intrauterine insemination cycles: An RCT. International Journal of Reproductive BioMedicine. Hoda Fazaely.

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Centrifuge: Principle Instrumentation and Applications ...

Centrifuge: Principle Instrumentation and Applications ...

When your centrifugation is complete, you should be able to see your biological specimen at the bottom of the tube in a pellet, which has separated from the rest of the solution, or supernatant. The supernatant can be removed by either decanting it – a fancy name for pouring it off, or it can be aspirated – a fancy term for using suction to ...

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