Send Email To Us

AC16 Human Cardiomyocyte Cell Line

AC16 Human Cardiomyocyte Cell Line

AC16 is a proliferating human cardiomyocyte cell line that s wa derived from the fusion of primary cells from adult human ventricular heart tissues with SV40 transformed, uridine auxotroph human fibroblasts, devoid of mitochondrial DNA (1, 2). After selection in uridinefree medium to remove unfused fibroblasts, -

Get Details

Quantitative Analysis of Chromatin Proteomes in Disease

Quantitative Analysis of Chromatin Proteomes in Disease

Adult mammalian cardiomyocyte nuclei ... Fill 1.5 ml centrifuge tube with 1 ml of sucrose buffer (24% sucrose weight/volume, 10 mM Tris pH 7.5, and 15 mM NaCl in deionized water with protease/phosphatase inhibitor mixture - sucrose buffer should be made fresh on the day of use). Gently layer the resuspended pellet on

Get Details

(PDF) Chromatin Immunoprecipitation of Adult Murine ...

(PDF) Chromatin Immunoprecipitation of Adult Murine ...

Adult mouse cardiomyocyte isolation is challenging and often does not provide. the required number of cells. In this unit we present a detailed protocol for cardiomyocyte ...

Get Details

A Mammalian Myocardial Cell-Free ... - Circulation Research

A Mammalian Myocardial Cell-Free ... - Circulation Research

Aug 06, 1999 Chemically isolated cardiomyocyte nuclei (1 to 1.510 5 neonatal, 0.5 to 110 5 adult) were immobilized onto poly-l-lysine (Sigma)–coated coverslips by centrifugation for 8 minutes at 600 rpm and 4C in a Beckman CS-6R centrifuge.

Get Details

Culture of Human Cardiomyocytes and its Applications in ...

Culture of Human Cardiomyocytes and its Applications in ...

Aug 27, 2021 Cardiomyocytes, or cardiac muscle cells are the main type of heart cells and act as the motor unit to drive heart contraction and relaxation. Particularly, adult human primary cardiomyocyte is a preferred in vitro model to evaluate drug-induced cardiotoxicity risk and develop new drugs for cardiac diseases [1].

Get Details

HL-1 Cells - Sigma-Aldrich

HL-1 Cells - Sigma-Aldrich

Centrifuge at 500g for 5 minutes. Meanwhile, remove the gelatin/fibronectin solution from each T25 flask, and add 4 mL supplemented Claycomb Medium/ flask. Set aside. Remove the tube containing the HL-cardiomyocytes from the centrifuge. Remove the supernatant by aspiration, and gently resuspend the pellet in 2 mL of supplemented Claycomb Medium.

Get Details

Modeling Cardiomyocyte Differentiation

Modeling Cardiomyocyte Differentiation

Centrifuge the 96-well V-bottom plate at 400 x g for 3 minutes. 17. Aspirate or quickly decant the primary antibody (or isotype control) solution. Resuspend the cells in 100 l/well permeabilization buffer. 18. Cover the plate with a lid and centrifuge at 400 x g for 3 minutes. 19. Repeat steps 17 and 18 two times to complete the wash. 20.

Get Details

Malonate Promotes Adult Cardiomyocyte Proliferation and ...

Malonate Promotes Adult Cardiomyocyte Proliferation and ...

Mar 05, 2021 Adult mice were injected intraperitoneally daily with either saline or 100 mg/kg dimethyl malonate after MI for 2 or 4 weeks. To track cardiomyocyte proliferation, we added 0.25 mg/mL 5-bromodeoxyuridine (Sigma) to the drinking water for 2 weeks after MI. Fresh bromodeoxyuridine-containing water was changed every 2 days.

Get Details

Isolation and in vitro culture of ... - Nature Protocols

Isolation and in vitro culture of ... - Nature Protocols

Mar 28, 2013 Adult mammalian cardiomyocytes proliferate insufficiently to regenerate injured myocardium; the damaged heart muscle is instead replaced by fibrotic scar tissue and the remaining cardiomyocytes...

Get Details

human adult cardiomyocyte phenotype Cardiovascular ...

human adult cardiomyocyte phenotype Cardiovascular ...

May 01, 2003 Abstract. Aim: Determination of the phenotype of adult human atrial and ventricular myocytes based on gene expression and morphology.Methods: Atrial and ventricular cardiomyocytes were obtained from patients undergoing cardiac surgery using a modified isolation procedure. Myocytes were isolated and cultured with or without serum. The relative …

Get Details

Isolation and culture of adult mouse cardiomyocytes for ...

Isolation and culture of adult mouse cardiomyocytes for ...

May 21, 2014 Add 5 ml diH 2 O to dissolve 1 g Na 2-ATP in 50 ml centrifuge tube, ... In summary, adult cardiomyocyte culture from genetically modified mice is a powerful tool for cardiac research. However, future work should keep in mind the genetic difference between humans and rodents when interpreting results.

Get Details

No Evidence for Cardiomyocyte Number Expansion in ...

No Evidence for Cardiomyocyte Number Expansion in ...

Nov 05, 2015 The magnitude of cardiomyocyte generation in the adult heart has been heavily debated. A recent report suggests that during mouse preadolescence, cardiomyocyte proliferation leads to a 40% increase in the number of cardiomyocytes. Such an expansion would change our understanding of heart growth and have far-reaching implications for cardiac ...

Get Details

Isolation Culture and Transduction of Adult Mouse ...

Isolation Culture and Transduction of Adult Mouse ...

Presented here, is a step-by-step method for the isolation and long-term culture of adult mouse cardiomyocytes. First, retrograde Langendorff perfusion is used to efficiently digest the heart with proteases, followed by gravity sedimentation purification.

Get Details

Circulating cardiomyocyte-derived extracellular vesicles ...

Circulating cardiomyocyte-derived extracellular vesicles ...

Primary adult mouse cardiomyocyte culture Mice were euthanized by carbon dioxide inhalation. The chest was opened, and the heart was rapidly removed and placed in an ice-cold HBBS solution (Thermo Scientic, USA). Cell isolation was performed based on a previously published protocol [11] with minor modication. The tissue

Get Details

Protocol for Imaging of Mitoflashes in Live Cardiomyocytes ...

Protocol for Imaging of Mitoflashes in Live Cardiomyocytes ...

Sep 18, 2020 Note: This protocol is primarily for adult cardiomyocytes. Cardiomyocyte cell lines and other cell types can also be assessed following this protocol. We have validated this protocol on neonatal cardiomyocytes, H9c2 cells, and A549 cells and NSC-34 cells. The cell number, centrifuge speed, and time are variable for different cell types.

Get Details

The 21-day postnatal rat ventricular cardiac muscle cell ...

The 21-day postnatal rat ventricular cardiac muscle cell ...

system for the study of adult cardiomyocyte gene expression and cell cycle machinery. (Mol Cell Biochem 229: 51–62, 2002) Key words: adult ventricular cardiomyocytes, gene expression, transfection, microinjection, cell cycle arrays tunately, the available experimental systems to study these phenomena remain limited.

Get Details

Adult Cardiomyocyte Cell Cycle Detour: Off-ramp to ...

Adult Cardiomyocyte Cell Cycle Detour: Off-ramp to ...

The Janus-Faced Cardiomyocyte: Deceptively Progressive Numerous Approaches for Assessing Mitosis: Most Inauthentic All sorts of results havebeen reported in the adult mammalian context with widely varying obser-vations of cardiomyocyte‘proliferation’ using a plethora of markers and metrics to assess de novo cardiomyogenesis [34,40,41].

Get Details

Thermo Scientific Pierce Primary Cardiomyocyte Isolation ...

Thermo Scientific Pierce Primary Cardiomyocyte Isolation ...

The Pierce Primary Cardiomyocyte Isolation Kit provides fully optimized reagents and a protocol that prevents overdigestion for the isolation and culture of cardiomyocytes. The complete process from handling primary tissues to seeding cells in culture vessels can be completed within 2 hours. Because it uses optimized tissue-specific ...

Get Details

(PDF) Isolation and Culture of Adult Mouse Cardiomyocytes ...

(PDF) Isolation and Culture of Adult Mouse Cardiomyocytes ...

Then centrifuge for 3 min at 20 x g to separate out small non-myocyte cells such as endothelial cells and fibroblasts. 6. Aspirate off the supernatant and resuspend the myocyte pellet in 10 ml calcium solution I with a supplement of 100 μl of 200 mM ATP (pH 7.2, stock solution, filter sterilized). ... In summary, adult cardiomyocyte culture ...

Get Details
Related Posts
<script type="text/javascript" src="https://s9.cnzz.com/z_stat.php?id=1281041249&web_id=1281041249"></script>